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difco macconkey agar base  (Difco)

 
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    Structured Review

    Difco difco macconkey agar base
    (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and <t>MacConkey</t> maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.
    Difco Macconkey Agar Base, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/difco+macconkey+agar+base/pmc12952977-46-0-0?v=Difco
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    difco macconkey agar base - by Bioz Stars, 2026-08
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    Images

    1) Product Images from "A prophage-expressed type IV pilus component provides anti-phage defense"

    Article Title: A prophage-expressed type IV pilus component provides anti-phage defense

    Journal: Cell reports

    doi: 10.1016/j.celrep.2025.116759

    (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and MacConkey maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.
    Figure Legend Snippet: (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and MacConkey maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.

    Techniques Used: Expressing, Fluorescence, Microscopy, Immunolabeling, Labeling



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    (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and <t>MacConkey</t> maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.
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    (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and <t>MacConkey</t> maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.
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    (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and <t>MacConkey</t> maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.
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    (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and <t>MacConkey</t> maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.
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    Image Search Results


    (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and MacConkey maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.

    Journal: Cell reports

    Article Title: A prophage-expressed type IV pilus component provides anti-phage defense

    doi: 10.1016/j.celrep.2025.116759

    Figure Lengend Snippet: (A) BacTH assays were performed by co-expressing JBD68 P-FimU or Pae strain PAO1 FimU (T25 fusion) with the minor pilin proteins, PilA, and T4P motor proteins PilU and PilB (T18 fusion). Interactions were detected by spotting cells on X-Gal and MacConkey maltose agar. (B) Percentage of cells making T4P in indicated strains as detected by fluorescence microscopy is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined by Tukey’s multiple comparisons test. (C) Percentage of cells with polar immunofluore-scence signal in the indicated strains is shown. Each data point represents a biological replicate, and the bar graphs indicate the mean ± SD. Statistics were determined using Student’s t test. ns, not significant. (D) Fluorescence microscopy images of cells from populations of indicated strains are shown with immunolabeled FimU and P-FimU (magenta) associated with T4P (green) labeled with AF488-maleimide. Arrows indicate pole- and pilus-associated FimU. Scale bars, 2 μm.

    Article Snippet: Difco MacConkey Agar Base , BD , Cat# 281810.

    Techniques: Expressing, Fluorescence, Microscopy, Immunolabeling, Labeling